Coding

Part:BBa_K4949005:Design

Designed by: UMananitoba_2023   Group: iGEM23_UManitoba   (2023-10-10)


lpp-OmpA-Est119


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 433
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression in E.coli


Source

Est119 is an esterase originally identified in the Thermobifida alba strain AHK119 (AB298783).

References